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Literature summary extracted from

  • Xu, Z.; Mulchandani, A.; Chen, W.
    Detection of benzene, toluene, ethyl benzene, and xylenes (BTEX) using toluene dioxygenase-peroxidase coupling reactions (2003), Biotechnol. Prog., 19, 1812-1815.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.12.11 analysis whole cell bioassay for the detection of benzene, toluene, ethyl benzene, and xylenes (BTEX) Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.11 expressed in Escherichia coli DHalpha Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.12.11 benzene + NADH + O2 Pseudomonas putida involved in the conversion of aromatic compounds to their corresponding catechols benzene dihydrodiol + NAD+
-
 ?
1.14.12.11 ethylbenzene + NADH + O2 Pseudomonas putida involved in the conversion of aromatic compounds to their corresponding catechols ?
-
 ?
1.14.12.11 toluene + NADH + O2 Pseudomonas putida involved in the conversion of aromatic compounds to their corresponding catechols (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?
1.14.12.11 xylene + NADH + O2 Pseudomonas putida involved in the conversion of aromatic compounds to their corresponding catechols ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.11 Pseudomonas putida
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.11 benzene + NADH + O2
-
 Pseudomonas putida benzene dihydrodiol + NAD+
-
 ?
1.14.12.11 benzene + NADH + O2 involved in the conversion of aromatic compounds to their corresponding catechols Pseudomonas putida benzene dihydrodiol + NAD+
-
 ?
1.14.12.11 ethylbenzene + NADH + O2
-
 Pseudomonas putida ?
-
 ?
1.14.12.11 ethylbenzene + NADH + O2 involved in the conversion of aromatic compounds to their corresponding catechols Pseudomonas putida ?
-
 ?
1.14.12.11 toluene + NADH + O2
-
 Pseudomonas putida (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?
1.14.12.11 toluene + NADH + O2 involved in the conversion of aromatic compounds to their corresponding catechols Pseudomonas putida (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?
1.14.12.11 xylene + NADH + O2
-
 Pseudomonas putida ?
-
 ?
1.14.12.11 xylene + NADH + O2 involved in the conversion of aromatic compounds to their corresponding catechols Pseudomonas putida ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.11 NADH
-
 Pseudomonas putida